Abstract: Rice is one of the most important food crops on earth, and the effective processing of its protein is of great significance. Using commercially available broken rice as raw material, the rice protein was separated and prepared by the alkaline method. The isoelectric point of the rice protein prepared by the alkaline method was determined, and the effects of factors such as the liquid-to-material ratio, reaction pH, reaction temperature, and reaction time on the extraction rate of rice protein were investigated, and the process conditions were optimized. The results showed that the optimal process conditions for preparing rice protein by the alkali method were a liquid-to-solid ratio of 1:8, a reaction pH of 11.5, a reaction temperature of 45 ° C, and a reaction time of 1.0 h. Under these conditions, the extraction yield of rice protein was 67%. Rice is an important food crop. According to statistics, about 50% of the world's population rely on rice as their staple food,...